Stem Barks of Bauhinia Acuminata | Analysis

Stem Barks of Bauhinia Acuminata | Analysis Materials and Methods Plant collection The stem barks of Bauhinia acuminata L. were collected from Rajshahi university campus, Bangladesh, in the month of September, 2013. The plant was authenticated by a taxonomist of Department of Botany, University of Rajshahi. A voucher specimen (Voucher No. MN-13) was deposited to the herbarium in the Department of Botany, University of Rajshahi. The stem barks were then washed separately with fresh water to remove dirty materials and were shade dried for several days with occasional sun drying. The dried barks were then ground into coarse powder by grinding machine and the materials were stored in dark at room temperature for future use. Extract preparation The extraction was performed according to method described by Alam el al. (Alam et al., 2002). About 400 gm of dried powdered stem bark were taken in an amber colored reagent bottle (2.5-liter capacity) and the materials were soaked in 2.0 liter of 100% methanol. The bottle with its contents were sealed and kept for a period of about 7 days with occasional shaking and stirring. The whole mixture was then filtered through cotton and then through Whatman No.1 filters paper and were concentrated with a rotary evaporator (Bibby Sterlin Ltd, UK) under reduced pressure at 45ÂÂ °C temperature to afford crude extract of the bark. The crude extract was mixed with 90% methanol in water to obtain a slurry of satisfactory volume of 100ml. The slurry was taken in a separating funnel and added equal amount of different partitioning solvent sequentially as like n-hexane, chloroform, ethyl acetate and finally water. The funnel was shaken vigorously and allowed to stand for a few minutes for sep aration of the compounds according to their partition co-efficient and thus extracts were prepared for the experiment purpose. The process was repeated three times. At last, the different fractionated parts were evaporated using rotary evaporator at 400C to obtain n-hexane fraction (NHF, 1.12 gm), chloroform fraction (CHF, 1.33 gm), ethyl acetate fraction (EAF, 2.27 gm) and aqueous fraction (AQF, 8.86 gm) respectively. Chemicals 1,1-diphenyl-2-picrylhydrazyl (DPPH), potassium ferricyanide, catechin (CA), ferrous ammonium sulphate, butylated hydroxytoluene (BHT), gallic acid (GA), ascorbic acid (AA), AlCl3, trichloro acetic acid (TCA), sodium phosphate, sodium nitrate, ammonium molybdate, 2-deoxy ribose, sodium hydroxide, EDTA and FeCl3 were purchased from Sigma Chemical Co. (St. Louis, MO, USA); potassium acetate, phosphate buffer, thiobarbituric acid(TBA),ÂÂ   HCl, H2SO4, H2O2were purchased from Sigma-Aldrich, vinblastine sulphate (VBS) from Cipla India, folin-ciocalteuss phenol reagent and sodium carbonate were obtained from Merck (Dam-stadt, Germany). Determination of total phenolics Total phenolic contents in the extracts were determined by the Folin-Ciocalteu method described by Singleton et al. (Slinkard Singleton, 1977). 40 ÂÂ µl of the extract/fractions (250ÂÂ µg/ml) were taken in test tubes and to each 3.16 ml of water was added to make up the volume 3.2ml. 200 ÂÂ µl of folin-Ciocalteu (Undiluted) reagent solution was added into the test tubes and kept for 5-8min. 600 ÂÂ µl of sodium carbonate (20%) solution was added into the test tubes and shake to mix. The test tubes were incubated for 2 hours at 20ËÅ ¡C to complete the reaction. Then the absorbance of the solution was measured at 765 nm using a spectrophotometer (Shimadzu, USA) against blank solution. A typical blank solution contains all reagents except plant extract or standard solution. Standard Gallic acid solutions (50-250ÃŽÂ ¼g /ml) concentrations were also treated as above. The total content of phenolic compounds in plant methanol extract and in different fractions wa s expressed as Gallic acid equivalent (GAE)/gm of dry extract in respect to standard gallic acid curve equation (y = 0.0008x 0.005, RÂÂ ² = 0.975). Determination of total flavonoids Total flavonoids were estimated using aluminum chloride colorimetric assay described by Zhishen et al. (Zhishen, Mengcheng, Jianming, 1999). To 0.5 ml of samples/standard, 150 ÂÂ µl of 5% sodium nitrate and 2.5 ml of distilled water were added. After 5 min, 0.3 ml of 10% AlCl3 was added. At 6 min, 1 ml of 0.001M NaOH and 0.55 ml distilled water was added to the mixture and left at RT for 15 min.ÂÂ   Absorbance of the mixtures was measured at 510 nm. Total flavonoid contents were expressed in terms of catechin equivalent, CAE /gm of dry extract in respect to standard curve equation (y = 0.0178x+0.0524, RÂÂ ² = 0.9862). Determination of free radical scavenging activity DPPH radical scavenging activity Free radical scavenging ability of the extracts was tested by DPPH radical scavenging assay (DRSA) as described by Braca et al. (Braca et al., 2001). Aliquots of 2.5mL of methanolic solution containing sample at different concentration was mixed with 2.5 ml of 0.008% DPPH solution in methanol. The reaction mixture was vortexed thoroughly and left in the dark at room temperature for 30 minutes. The absorbance of the mixture was measured spectrophotometrically at 517 nm. Ascorbic acid was used as reference. Percentage DPPH radical scavenging activity (% DRSA) was calculated by the following equation, % DRSA = (A0-A1/A0) x 100 Where, A0 = Absorbance of control and A1 = Absorbance of sample. IC50 values denote the concentration of sample required to scavenge 50% DPPH free radicals. Hydrogen peroxide scavenging activity Hydrogen peroxide scavenging activity of extract was assessed by the method described by Zhang et al. (Zhang He). Aliquot of 1.0 ml of 0.1mmol/L H2O2 and 1.0 ml of various concentrations of extracts were mixed. Followed by 2 drops of 3% ammonium molybdate, 10 ml of 2M H2SO4 and 7.0 ml of 1.8 mol/L KI. The mixed solution was titrated with 5.09mmol/L Na2S2O3 until yellow color disappeared. The extent of scavenging of hydrogen peroxide was calculated as: % scavenging of hydrogen peroxide = [(V0-V1) / V0] ÃÆ'- 100 Where, V0 = Volume of Na2S2O3 solution used to titrate the control sample in the presence of hydrogen peroxide (without sample), V1 = Volume of Na2S2O3 solution used in the presence of samples. Hydroxyl radical scavenging activity Hydroxyl radical scavenging activity (HRSA) of the extracts was determined by the method of Halliwell et al. (Halliwell, Gutteridge, Aruoma, 1987) with a slight modification. 100 ÂÂ µl of various concentrations of extracts were mixed with 100 ÂÂ µl of hydrogen peroxide (10mmol/L). To this 200 ÂÂ µl premixed FeCl3 (100mmol/L) and EDTA (100mmol/L) solution (1:1;v/v) was added. Followed by addition of 500 ÂÂ µl of 2.8mmol/L 2-deoxyribose in phosphate buffer (PH7.4) and finally the reaction was triggered by adding 100 ÂÂ µl ascorbate (300mmol/L).ÂÂ   Then the reaction mixture was incubated at 37.5ËÅ ¡C for 1 hour. To the above reaction mixture 2 ml of TCA (2.8% w/v aqueous solution) and 2 ml of TBA (1% w/v aqueous solution) was added. The final reaction mixture was heated for 15min in boiling water bath, cooled and absorbance was taken at 532nm using a spectrophotometer. The hydroxyl radical scavenging capacity was evaluated with the inhibition percentage of 2-deoxyribose oxidation on hydroxyl radicals. The percentage of hydroxyl radical scavenging (%HRSA) activity was calculated according to the following formula: % HRSA = [A0 (A1-A2] ÃÆ'-100/A0 where A0 is the absorbance of the control without a sample. A1 is the absorbance after adding the sample and 2-deoxyribose. A2 is the absorbance of the sample without 2-deoxyribose. REFERENCES Alam, A., Rahman, M., Baki, M., Rashid, M., Bhuyan, M., Sadik, G. (2002). Antidiarrhoeal principle of Achyranthes ferruginea Roxb. and their cytotoxicity. Ban Pharm J, 12, 1-4. Braca, A., De Tommasi, N., Di Bari, L., Pizza, C., Politi, M., Morelli, I. (2001). Antioxidant Principles from Bauhinia tarapotensis. Journal of Natural Products, 64(7), 892-895. doi:10.1021/np0100845 Halliwell, B., Gutteridge, J. M., Aruoma, O. I. (1987). The deoxyribose method: a simple test-tube assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem, 165(1), 215-219. Slinkard, K., Singleton, V. L. (1977). Total Phenol Analysis: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture, 28(1), 49-55. Zhang, X., He, F. Science Press; Beijing, China: 2000. Principle of Chemical Analysis, 275-276. Zhishen, J., Mengcheng, T., Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food chemistry, 64(4), 555-559.

Indifference to War in Remarque’s All Quiet on the Western Front Essay

Indifference to War in Remarque’s All Quiet on the Western Front In Remarque’s All Quiet on the Western Front, characters such as Paul and his friends become indifferent to shocking elements of war through constant exposure to them. For example, the characters are unconcerned about the dangers of the front because they are accustomed to being on the front. In another instance, Paul’s friends show no emotions when they witness snipers killing enemy soldiers. Also, Kat finds the unusual effects of mortar shells amusing. These examples prove that through war, characters of the book have become indifferent to things that they would normally find shocking. Many characters on the front are indifferent to the dangers of the front. They are not concerned about near death e...

Visual Merchandising

Visual Merchandising: Visual Merchandising is very important because the first impression of the product or store on the customer is by visualizing. If the impression is positive then customers attract to the store. A store must have the inviting appearance to the customers. Objectives of Visual Merchandising: Objective of visual merchandise is to attract the customers to your business in order to sell the merchandise. The main objective is to make the customers feel comfortable and eager to buy. Essential of Visual Merchandising: * Interior Floor  Design  and Display. * Space and Signage. * Window Display. Tools Use for Visual Merchandising: Audio-Visual Displays. * Decoration and Props. * Signage and Graphics. The presentation in visual merchandising falls into two categories mainly: 1. Exterior Presentations. 2. Interior Presentations. Exterior Presentations: The outlook of a store is a major determinant for a customer. Good exterior presentation attracts attention, creates in terest and invites the customer into business. It involves exterior signs, banners and window display. Exterior Signs: A store sign is its â€Å"signature† which tells the customers that what type of the business and what it sells. In less than 10sec the sign must attract attention.Banners: Banners are used to sales promotion. It should be very colorful and eye catching. Window Display: Some products should be placed on a store’s window. Window display should attract attention, create interest and invite people into the store to purchase goods. New displays indicate new up-to-date merchandise is available. Interior Presentations: Another way of the merchandising is through interior display that effectively show merchandise to the customers. The purpose of doing this is to develop interest for the merchandise. It involves color theme and images, lighting, props and fixtures. Researchers found that 64. % of all purchase decision was made inside a store. Three goals of st ore are- 1. Motivate the customers to spend money 2. Protect the image of the store 3. Keep expenses to minimum Color and Lighting: Color in a display can catch eye and make people pause and look. The overall color combination can affect the atmosphere of a store. Lighting is essential in calling attention to merchandise in a display. Customer’s eye is drawn automatically to the brightest area. Props and Fixtures: A display prop is not for sale, it is just used with a product in a display to clarify the function of the product being sold.Merchandise and Fixture Display Recommendations: Goods can be effectively displayed on a variety of fixtures such as tables, cubes, racks and other display cases. Movable shelves from all sides used in self-service retail stores to display merchandise. They can be lined up in stores as grocery or hardware stores. Errors Commonly Occurring in Display: * Too much merchandise * Too little merchandise * Too many props * Poorly selected props * Di splay changed to seldom * Limited or no display budget * Lack of attention to detail

Ap Gov Unit 3 - 1006 Words

What important purposes are served by the right to amend the Constitution guaranteed by Article V? Evaluate James Madison’s claim in Federalist 43 that the Constitution â€Å"guards equally against the extreme facility which would render the Constitution too mutable, and that extreme difficulty which might perpetuate its discovered faults.† Some critics of the amending process contend that amendments, once proposed, should be submitted to popular vote, bypassing state legislatures. Do you agree or disagree? Why? P1. According to professor Douglas Linder, the delegates assembled in Philadelphia were under no illusions that the constitutional scheme they were struggling to establish was perfect for present circumstances, much less perfect†¦show more content†¦According to John Locke, the government derives its power from the people. Choosing to bypass state legislatures and resorting to state conventions forces people to participate in government and have a direct say in laws that affect them. The creation of the constitution is intended to uphold the concept behind limited government and promote civic virtue. P.7 With the end of the Civil War and the 14th Amendment’s creation of a national citizenship, the Court has found that most of the rights recognized in the Bill of Rights apply to state constitutions through the due process clause. Beginning in the 1920s and most recently in 2011 the Court has used this amendment to amend state constitutions through a process that Ma dison most likely could not have envisioned in 1787. P.8 Since 1789, members of Congress have proposed over 10,000 amendments. Only 33 have passed through Congress, and of those only 27 have been ratified by the states. Madison was right about the difficulty of the amendment process; what he could not have foreseen was the Court’s use of an amendment to expand the rights originally demanded to beShow MoreRelatedApproved EPR Bullets9088 Words   |  37 Pagesï » ¿PRIMARY/ADDITIONAL DUTIES - Deplyd as Unit Intel Liaison to Eskan Village, Saudi Arabia; performed AT/FP f/$1B in AF assets--zero loss - Prepd/conducted 20 jt Intel briefs w/OSI; 174 SF mbrs current on threat--1.2K mbrs/three commands secured - Provided background checks f/50+ personnel/125 hrs/coordd w/OSI--alleviated screening process deficiency - Sustainment firing program administrator; personally trnd/certd 50 SF Amn--maintained critical weapon certs - Deplyd to Thumrait AB, Oman; shielddRead MoreBank of Queensland Research Paper Banking Industry21330 Words   |  86 Pages........ .................. .................. ....................................................  3   1.  0  Profiling  the e  Corporate e  Structure     discussin ng  the  Agency  problem m  ..................................................  3   Overview  .................... O .................. .................. .................. .................. ....................................................  3   Core operat C ting activiti .............. ies   .................. .................. ............Read MoreMedicare Policy Analysis447966 Words   |  1792 Pagesassembled, 3 SECTION 1. SHORT TITLE; TABLE OF DIVISIONS, TITLES, rmajette on DSK29S0YB1PROD with BILLS 4 5 AND SUBTITLES. (a) SHORT TITLE.—This Act may be cited as the 6 ‘‘Affordable Health Care for America Act’’. VerDate Nov 24 2008 12:56 Oct 30, 2009 Jkt 089200 PO 00000 Frm 00001 Fmt 6652 Sfmt 6201 E:\BILLS\H3962.IH H3962 2 1 2 (b) TABLE TITLES.—This OF DIVISIONS, TITLES, AND SUB- Act is divided into divisions, titles, and 3 subtitlesRead MoreProject Mgmt296381 Words   |  1186 Pagesprojects 2.3 Stakeholders and review boards 12.1 RFP’s and vendor selection (.3.4.5) 11.2.2.6 SWAT analysis 6.5.2.7 Schedule compression 9.4.2.5 Leadership skills G.1 Project leadership 10.1 Stakeholder management Chapter 11 Teams Chapter 3 Organization: Structure and Culture 2.4.1 Organization cultures [G.7] 2.4.2 Organization structure [9.1.3] 9.1.1 Organization charts 1.4.4 Project offices Chapter 4 9.2 Building the team (.1.3) [3.5.3] [App G.2 Building teams] 9.4 ManagingRead MoreProject Management Infomaton System48477 Words   |  194 Pagesof DME-IS ........................................ 19 2.5 DME-IS levels of Technology ................................... 20 2.6 Identifying a project’s MIS technology needs ............. 26 2.7 From Data to Information to Knowledge and Wisdom .. 29 3. FUNDAMENTALS OF DME-IS ............................................... 33 3.1 The Core Processes of DME-IS.................................. 33 3.2 Managing the Information Process ............................ 35 3.3 Depth of detail in DME-IS (keepRead MoreLibrary Management204752 Words   |  820 PagesLibrary and information center management / Robert D. Stueart and Barbara B. Moran. — 7th ed. p. cm. — (Library and information science text series) Includes bibliographical references and index. ISBN 978–1–59158–408–7 (alk. paper) ISBN 978à ¢â‚¬â€œ1–59158–406–3 (pbk. : alk. paper) 1. Library administration—United States. 2. Information services— United States—Management. I. Moran, Barbara B. II. Title. Z678.S799 2007 025.1—dc22 2007007922 British Library Cataloguing in Publication Data is available. CopyrightRead MoreStephen P. Robbins Timothy A. Judge (2011) Organizational Behaviour 15th Edition New Jersey: Prentice Hall393164 Words   |  1573 Pages658.3—dc23 2011038674 10 9 8 7 6 5 4 3 2 1 ISBN 10: 0-13-283487-1 ISBN 13: 978-0-13-283487-2 Brief Contents Preface xxii 1 2 Introduction 1 What Is Organizational Behavior? 3 The Individual 2 3 4 5 6 7 8 Diversity in Organizations 39 Attitudes and Job Satisfaction 69 Emotions and Moods 97 Personality and Values 131 Perception and Individual Decision Making 165 Motivation Concepts 201 Motivation: From Concepts to Applications 239 3 The Group 9 10 11 12 13 14 15 FoundationsRead More_x000C_Introduction to Statistics and Data Analysis355457 Words   |  1422 Pagespermission to use material from this text or product, submit a request online at http://www.thomsonrights.com. Any additional questions about permissions can be submitted by e-mail to thomsonrights@thomson.com. Printed in the United States of America 1 2 3 4 5 6 7 11 10 09 08 07 ExamView  ® and ExamView Pro  ® are registered trademarks of FSCreations, Inc. Windows is a registered trademark of the Microsoft Corporation used herein under license. Macintosh and Power Macintosh are registered trademarks of AppleRead MoreCorporation (Fisch) Outline Penn Law Essay62808 Words   |  252 PagesDuty of Loyalty 56 3.) Duty of Fairness: Parent-Subsidiary Relationships 63 4.) Duty of Good Faith 64 5.) Management Obligations Under Federal Securities Laws 67 C.) Shareholder Litigation 76 IV.) Structural Changes 85 A.) Transactions in Control 85 B.) Mergers and Acquisitions 86 1.) Mergers 87 2.) Sale of Assets 93 3.) Asset Purchase or Tender Offer 94 C.) Public Control Contests 96 1.) The Poison Pill 100 2.) Enhanced Review When Business is Up for Sale 103 3.) Proxy Contests forRead MoreCase Studies: Sas Airline Ryanair80169 Words   |  321 Pages................................................................................. 1 1.2 RESEARCH PROBLEM............................................................................................................................................... 3 1.3 RESEARCH OBJECTIVES ........................................................................................................................................... 4 1.4 DEMARCATIONS AND LIMITATIONS OF THE STUDY....................................